Iodixanol as a density gradient medium for the isolation of motile spermatozoa
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چکیده
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OptiPrepTM The ideal density gradient medium for isolation of cells
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15 صفحه اولMotility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, ...
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The flotation of the different classes of human plasma lipoproteins in a centrifugal field by the sequential elevation of the density of the plasma by dissolution of KBr or NaCl/KBr mixtures has been widely used over many years. The operation is routinely carried out in a fixed-angle rotor and takes at least three days. In spite of its cumbersome nature, the technique is still widely used today.
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 1997
ISSN: 1058-0468,1573-7330
DOI: 10.1007/bf02766145